Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8384124 | FEBS Letters | 2008 | 8 Pages |
Abstract
Macrophage clearance of dying cells is of crucial importance to maintain tissue homeostasis. Here, we show that brief treatment (15 min) of Jurkat T cells with agonistic anti-Fas antibodies or recombinant Fas ligand results in efficient phagocytosis by human monocyte-derived macrophages prior to the occurrence of common biomarkers of apoptosis. Similar findings were obtained when using primary human T cells. Macrophage engulfment of pre-apoptotic target cells was suppressed in the absence of serum. Moreover, pre-apoptotic cells secreted annexin I and administration of Boc1, a formyl peptide receptor/lipoxin receptor antagonist markedly attenuated their engulfment. Finally, pre-apoptotic Jurkat cells induced lower macrophage production of tumor necrosis factor-α and higher production of interleukin-10 in comparison to apoptotic target cells.
Keywords
FBSDEVD-AMCannexin IzVAD-fmkHMDMFPRL-1M-CSFPARPDAPICTL4′-6-diamidino-2-phenylindoleinterleukinTamratumor necrosis factor-αfetal bovine serumCytokineTNF-αPhagocytosisPhosphatidylserineCytotoxic T lymphocytesmacrophage colony-stimulating factorhuman monocyte-derived macrophagesPoly(ADP-ribose) polymerase
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Authors
Shouting Zhang, Erika Witasp, Marjolein Lauwen, Bengt Fadeel,