Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8384134 | FEBS Letters | 2008 | 5 Pages |
Abstract
To find novel proteins predicted to participate in the formation of nuclear bodies, nuclear speckles, and nuclear macro-protein complexes, we applied proteome analysis to a HeLa cell nuclear matrix fraction. Proteins in the fraction were separated by SDS-PAGE, digested with trypsin, and analyzed by nanoflow liquid chromatography-iontrap-tandem mass spectrometry. Three hundred and thirty three proteins including 39 novel ones were identified. Seven WD-repeat proteins and 16 disordered region-rich proteins, which act frequently as scaffolding proteins for macro-protein complexes, were found amongst the novel proteins.
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Authors
Kohei Ishii, Yasuhiro Hirano, Nanase Araki, Toshiyuki Oda, Masahiro Kumeta, Kunio Takeyasu, Kazuhiro Furukawa, Tsuneyoshi Horigome,