Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8386897 | Journal of Plant Physiology | 2018 | 31 Pages |
Abstract
Alpha-momorcharin (α-MMC) is a type-I ribosome inactivating protein (RIP) with a molecular weight of 29â¯kDa found in plants. This protein has been shown to be effective against a broad range of human viruses and also has anti-tumor activities. However, the mechanism by which α-MMC induces plant defense responses and regulates the N gene to promote resistance to the Tobacco mosaic virus (TMV) is still not clear. By using pharmacological and infection experiments, we found that α-MMC enhances TMV resistance of tobacco plants containing the N gene (tobaccoNN). Our results showed that plants pretreated with 0.5â¯mg/ml α-MMC could relieve TMV-induced oxidative damage, had enhanced the expression of the N gene and increased biosynthesis of jasmonic acid (JA) and salicylic acid (SA). Moreover, transcription of JA and SA signaling pathway genes were increased, and their expression persisted for a longer period of time in plants pretreated with α-MMC compared with those pretreated with water. Importantly, exogenous application of 1-Aminobenzotriazole (ABT, SA inhibitor) and ibuprofen (JA inhibitor) reduced α-MMC induced plant resistance under viral infection. Thus, our results revealed that α-MMC enhances TMV resistance of tobaccoNN plants by manipulating JA-SA crosstalk.
Keywords
EDS1NBTΦPSIITMVABTNPQNPR1alpha-momorcharinCOI1PODRbohDPDF1.2ICS1Elongation factor 1AMDAquantum efficiency of PSIIplant defensin 1.2DABCAThpiAPXqRT-PCR1-aminobenzotriazole3,3-diaminobenzidineEF1αFv/Fmjasmonic acidSalicylic acidAOSnitro blue tetrazoliumcoronatine insensitive 1Non-photochemical quenchinghours post-inoculationSODAllene oxide synthaseSuperoxide dismutasemalondialdehydequantitative real-time PCRTobacco mosaic virusRIPHypersensitive responsePeroxidaseascorbate peroxidaseRibosome-inactivating proteinN genePathogenesis-related geneCatalase
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Authors
Ting Yang, Li-sha Zhu, Yao Meng, Rui Lv, Zhuo Zhou, Lin Zhu, Hong-hui Lin, De-hui Xi,