Expression profiling of genes encoding ABA route components in response to dehydration or various light conditions in poplar buds and leaves

In buds, three studied Rboh genes took part in the early response to dehydration, however their participation involved the visibly highest level of the Ptre-RbohD1 transcripts, followed by Ptre-RbohF2 and the lowest one of Ptre-RbohF1. Nonetheless, the further stress-induced superoxide anion generation seemed to depend on the enhanced expression of the Ptre-RbohD1 and Ptre-RbohF2 genes only, still with a significantly higher level of the Ptre-RbohD1 one. Ptre-RbohD2 transcripts were found neither in leaves nor in buds. The expression of the other genes discussed in the present work was either slightly upregulated at moderate stress or did not significantly change in response to dehydration. The protein kinase activity of overexpressed Ptre-SnRK2.6a and Ptre-SnRK2.6b was confirmed in in vitro protein kinase assay and compared to that of SnRK2.6/OST1 one from Arabidopsis.