Development of a dual promoter-mediated gene silencing system in Flammulina velutipes

This study aimed to establish an RNAi system based on a dual promoter construct to interfere with the expression of target genes in Flammulina velutipes. In this study, the endogenous laccase gene, which was cloned as a silencing reporter, was introduced into F. velutipes through electroporation-mediated transformation. Our data unequivocally indicate that the dual promoter silencing system significantly reduced the expression and activity of lac6. Additionally, reductions in lac6 mRNA levels and enzyme activity were correlated (minimum of a 50% reduction). The molecular tools developed in this study should facilitate the functional characterization of genes in this important species.