Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8393560 | Systematic and Applied Microbiology | 2017 | 8 Pages |
Abstract
In this study, the effect of nitrogen addition on GAO carbon metabolism was investigated during an 8Â h cycle. Activated sludge dominated by GAO from a winery wastewater sequencing batch reactor was incubated under different carbon to nitrogen (COD:N) ratios (100:1, 60:1 and 20:1) using 13C - acetate and 15N - urea. GAO cell assimilation was quantified using FISH-NanoSIMS. The activated sludge community was assessed by 16S rRNA gene profiling, DNA and storage polymer production. Carbon and nitrogen quantification at the cellular level by NanoSIMS revealed that low (COD:N of 100:1) or null nitrogen concentrations enhanced GAO carbon uptake. COD:N ratios of 60:1 and 20:1 reduced GAO carbon uptake and promoted whole microbial community DNA production. Nitrogen dosing at COD:N ratios of 60:1 or higher was demonstrated as feasible strategy for controlling the excessive GAO growth in high COD waste treatment plants.
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Authors
Cristobal A. Onetto, Kathryn L. Eales, Paul Guagliardo, Matt R. Kilburn, Joanna M. Gambetta, Paul R. Grbin,