Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8403767 | Animal Reproduction Science | 2018 | 9 Pages |
Abstract
Cinnamtannin B-1 (CNB-1) is a naturally occurring trimeric A-type proanthocyanidin contained in several plants such as cinnamon (Cinnamomum zeylanicum). It is considered to be a potent antioxidant. The protective effect of CNB-1 against oxidative stress was assessed in red deer epididymal sperm incubated at 37â¯Â°C. Cryopreserved sperm from six stags were thawed, pooled and extended to 400â¯Ãâ¯106 sperm/ml in BGM (bovine gamete medium). After being aliquoted, the samples were supplemented with different concentrations of CNB-1 (0, 0.1, 1, 10 and 100â¯Î¼g/mL), with or without induced oxidative stress (100 μM Fe2+/ascorbate). The samples were evaluated after 0, 2 and 4â¯h of incubation at 37â¯Â°C. This experiment was replicated six times. Spermmotility (CASA), viability, mitochondrial membrane potential, acrosomal status, lipoperoxidation (C11 BODIPY 581/591), intracellular reactive oxygen species (ROS) production and DNA status (TUNEL) were assessed. After 4â¯h of incubation, CNB-1 prevented the deleterious effects of oxidative stress, thus improved sperm progressivity and velocity (P<0.05). Furthermore, 1 and 10 μM CNB-1 improved sperm linearity, even when compared to those samples that had not been subjected to oxidative stress (P<0.05). The greatest concentration, 100 μM, prevented sperm lipoperoxidation and reduced ROS production in samples subjected to oxidative stress.
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Animal Science and Zoology
Authors
F. Sánchez-Rubio, M.R Fernández-Santos, L. Castro-Vázquez, O. GarcÃa-Álvarez, A. Maroto-Morales, A.J. Soler, F. MartÃnez-Pastor, J.J. Garde,