Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8403862 | Animal Reproduction Science | 2018 | 28 Pages |
Abstract
Vitamin E is a potent molecule, especially when loaded in cyclodextrin, in modulating oxidative stress during the freeze-thawing process. The present study aimed to investigate the effect of different concentrations of cyclodextrin-vitamin E complex (CD-Vit E) on cryopreserved ram sperm. Ejaculates collected from five adult rams were pooled and divided into four aliquots. All aliquots were treated in Tris-extender (Tris-glucose-citric acid) containing 2â¯mg cholesterol-loaded methyl-β- cyclodextrin/120â¯Ãâ¯106 spermatozoa and either 0 (Control), 2, 4 or 6â¯mg CD-Vit E/120â¯Ãâ¯106 spermatozoa, corresponding to 0, 0.5, 1 or 1.5 of pure vitamin E, respectively. After incubation at 22â¯Â°C for 15â¯min and the addition of Tris-extender containing glycerol and egg yolk (v/v), all aliquots were frozen in liquid nitrogen. After thawing, motility (computer aided sperm analysis), viability (eosin staining), membrane integrity (HOST), acrosome integrity (Coomassie G-250 staining) and lipid peroxidation (Thiobarbituric acid assay) were evaluated. Compared to control, 2â¯mg CD-Vit E had a significant positive effect on total motility, progressive motility, movement linearity (LIN%), viability and lipid peroxidation. At 4â¯mg, however, CD-Vit E had a significant negative effect on total motility, progressive motility, membrane functionality and acrosome integrity. At a greater concentration (6â¯mg), the negative effects were greater as compared with inclusion of 4â¯mg in the cryoprotectant and the percentage of rapidly and moderately motile gametes and viability were also altered. In conclusion, the effect of CD-Vit E on cryopreserved ram sperm was concentration-dependent with the 2â¯mg amount having a beneficial effect while greater concentrations (4 and 6â¯mg) had a harmful effect on sperm motility and gamete integrity but without affecting oxidative stress status.
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Authors
Karim Benhenia, Hamza Rahab, Mustapha-Adnane Smadi, Hamza Benmakhlouf, Ali Lamara, Takfarinas Idres, Mokrane Iguer-Ouada,