Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8403938 | Animal Reproduction Science | 2018 | 28 Pages |
Abstract
The aim of the present study was to evaluate the effects of Percoll volume on recovery rate, sperm quality, and embryo development kinetics in in vitro production of cattle embryos. Straws of conventional and sex-sorted semen were allocated to three different volumes of Percoll: 300â¯Î¼L of each Percoll gradient (90%, 60%, and 30%), Control; 100â¯Î¼L of each Percoll gradient, P100; and 200â¯Î¼L of each Percoll gradient, P200. Sperm quality, fertilization rate, and embryo morpho-kinetic development using time lapse cinematography up to 48â¯h post-insemination were evaluated. For conventionally processed semen, sperm motility, vigor, and recovery rate were greater in the P100 and P200 treatment groups compared to the Control (Pâ¯<â¯0.05), whereas reactive oxygen species (ROS) concentrations, lipid peroxidation, and superoxide dismutase enzyme activity were not influenced by treatments. For sex-sorted semen, treatment with P100 increased sperm curvilinear velocity, average path velocity, and amplitude of lateral head displacement (Pâ¯<â¯0.05). Recovery rate was greater in the P100 group than Control and P200 groups (Pâ¯<â¯0.05), formation of ROS was less in the P100 than Control and P200 groups, and superoxide dismutase enzyme activity was less in the P100 than Control group. Fertilization and cleavage rates, time of first cleavage, and cell number were similar between the P100 and Control groups (Pâ¯>â¯0.05). The inclusion of Percoll volumes of 100â¯Î¼L resulted in an increased sperm recovery rate without damage to sperm quality or affecting early embryonic development.
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Authors
Daniele Missio, Natália Picolli Folchini, Fabio Gallas Leivas, CecÃlia Isabel Inês Urquiza Machado Pavin, Hirya Fernandes Pinto, Francielli Weber Santos Cibin, Daniela dos Santos Brum,