Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8403959 | Animal Reproduction Science | 2018 | 21 Pages |
Abstract
The quality of boar spermatozoa is affected by oxidative stress during preservation in vitro. It has been demonstrated that L-Glutamine (Gln) can effectively protect cells from oxidative stress-induced injury. There are, however, no reports to date evaluating the effects of Gln on boar semen liquid preservation at 17â¯Â°C. The aims of the present study were to elucidate whether the addition of Gln to the extender BTS could improve the quality of boar spermatozoa when stored at 17â¯Â°C and to determine the mechanism underlying Gln protection of spermatozoa against preservation-induced damage. Boar semen samples were collected and diluted with Beltsville Thawing Solution (BTS) containing different concentrations (0, 10, 20, 40 or 80â¯mM) of Gln. The results indicated the addition of 20â¯mM Gln to the BTS improved (Pâ¯<â¯0.05) the motility, acrosome integrity and membrane integrity of boar sperm during liquid preservation. Interestingly, treatment of spermatozoa with Gln addition to the extender resulted in ROS quenching, while enhancing γ-glutamyl cysteine synthetase (γ-GCS) activity, and glutathione (GSH) content of spermatozoa. These results suggest that BTS supplemented with Gln can provide greater protective capacity to boar sperm against oxidative stress by enhancing GSH synthesis during liquid preservation.
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Authors
Shunwei Wang, Meng Sun, Na Wang, Kang Yang, Haitao Guo, Jingran Wang, Yuting Zhang, Shunli Yue, Jiabo Zhou,