| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8404020 | Animal Reproduction Science | 2018 | 38 Pages |
Abstract
Luteinizing hormone (LH) is a glycoprotein that regulates the function of ovarian follicular cells. Theca cells (TCs) also have a key role in follicular growth and atresia. The effects and intracellular signaling mechanisms were investigated of LH on apoptosis and steroidogenesis in goat gonadotropin-independent follicular (1.0-4.0â¯mm) TCs. The results indicated that LH increased androstenedione secretion and relative abundance of CYP17A1 and BCL2 mRNA in the TCs, whereas LH in combination with LY294002, a PI3K/AKT inhibitor, decreased LH-induced function. The apoptosis ratio and expression of the BAX gene in TCs were less with LH treatment, and the extent of this inhibition was decreased by suppressing the PI3K/AKT pathway. In conclusion, results of the present study indicate LH regulates apoptosis and steroidogenesis in goat TCs by activating the PI3K/AKT pathway.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Animal Science and Zoology
Authors
Xiaomei Wang, Pengda Zou, Yuanyuan He, Kai Meng, Fusheng Quan, Yong Zhang,