Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8404297 | Animal Reproduction Science | 2015 | 7 Pages |
Abstract
The aims of this study were to evaluate the effects of anti-oxidant butylated hydroxytoluene (BHT), when added at different concentrations into lecithin-based Bioxcell® (BX) and two egg-yolk-based; Tris (TY) and citrate (CE) semen extenders, on post-thaw bull sperm quality and oxidative stress. A total of 30 ejaculates from three bulls were collected using an electro ejaculator. Ejaculates were extended with one of the BX, TY and CE extenders, which contained different concentrations (0.0 - control, 0.5, 1.0, 1.5, 2.0 and 3.0 mM/ml) of BHT. The extended semen samples were chilled to 4 °C, and then frozen slowly to â196 °C in 0.25 ml straws before being stored in liquid nitrogen for 2 weeks. Results showed that supplementation of BHT improved (P < 0.05) general motility, progressive motility, morphology, acrosome integrity, DNA integrity and malondialdehyde of sperm at 0.5 mM/ml for BX and at 1-1.5 mM/ml of BHT for TY and CE when compared with the control. However, greater concentrations of 2.0 and 3.0 mM/ml of BHT had a detrimental (P < 0.05) effect compared with the control with all extenders evaluated. In conclusion, BHT supplementation at lesser concentrations (0.5-1.5 mM/ml) could improve frozen-thawed bull sperm quality by reducing oxidative stress produced during the freezing-thawing procedures in either lecithin or egg-yolk based extenders.
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Authors
A.M. Khumran, N. Yimer, Y. Rosnina, M.O. Ariff, H. Wahid, Asmatullah Kaka, M. Ebrahimi, K. Sarsaifi,