Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8404676 | Animal Reproduction Science | 2014 | 6 Pages |
Abstract
Sperm-mediated gene transfer (SMGT) is a promising transgenic technology that relies on the capability of sperm to internalize exogenous DNA. In marine fish, however, the interaction between sperm and exogenous DNA appears to be deficient. Here, we demonstrated significant DNase activity in the seminal plasma of the olive flounder. When incubated with naked-DNA, the spermatozoa lost their structural integrity, including the head, mitochondria and flagellum, in an incubation time-dependent manner. However, internalization of a liposome-DNA complex resulted in the structural integrity of the spermatozoa being maintained, even when using incubation times of up to 50Â min. We concluded that in the olive flounder, SMGT is possible by integrating liposome-DNA complexes, rather than naked-DNA alone, into the sperm. In brief, removal of the seminal plasma and packaging the exogenous DNA were necessary for successful SMGT in the olive flounder.
Keywords
Related Topics
Life Sciences
Agricultural and Biological Sciences
Animal Science and Zoology
Authors
Nian Xin, Tiantian Liu, Haitao Zhao, Zhenwei Wang, Jinxiang Liu, Quanqi Zhang, Jie Qi,