Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8404899 | Animal Reproduction Science | 2014 | 5 Pages |
Abstract
This study evaluated associations between the presence of protein bands in ram seminal plasma and the quality of sperm frozen with distinct extenders. Ejaculates were frozen in a Tris-egg yolk based extender, including either 5% glycerol or 100Â mM trehalose. Seminal plasma samples were submitted to unidimensional electrophoresis. Pre-freezing and post-thawing sperm quality was similar between extenders (PÂ >Â 0.05). A total of 26 bands were identified in ram seminal plasma. Pre-freezing sperm motility was increased when the 15, 19 and 80Â kDa bands were present in seminal plasma (PÂ <Â 0.05). The presence of an 11Â kDa band in seminal plasma was associated with reduced pre-freezing membrane integrity (PÂ <Â 0.05). After thawing, both sperm motility and membrane integrity were reduced when a 24Â kDa band was present in seminal plasma (PÂ <Â 0.05). Post-thawing acrosome integrity was greater in the presence of a 31Â kDa band in seminal plasma (PÂ <Â 0.05). Regardless of the cryoprotectant included in the freezing extender, these six bands may be potential markers for ram sperm tolerance to freezing.
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Authors
K.L. Goularte, G.D.A. Gastal, R.S. Schiavon, A.O. Gonçalves, J.R. Schneider, C.D. Corcini, T. Jr.,