Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8405777 | Biocatalysis and Agricultural Biotechnology | 2018 | 34 Pages |
Abstract
The aim of the present study was to evaluate the mosquito larvicidal activity of lawsone isolated from Lawsonia inermis (L). The larvae were exposed to four different concentrations of lawsone (0.5, 1.0, 1.5 and 2.0â¯ppm). After 24â¯h and 120â¯h post treatment the larval mortality and ovicidal activity were recorded. However, Lawsone shows significant larvicidal activity against Culex quinquefasciatus with LC50 and LC90 values of 0.72 and 5.04â¯ppm along with 84% of ovicidal activity at 2â¯ppm concentration. Histopathological study revealed that serious damage on the larval midgut cells. Lawsone showed no harmful effect on the tested organism Poecilia reticulata. Lawsone was highly active and inhibited AChE in a concentration dependent manner. Lawsone presented highest inhibition against AChE extracted from Cx. quinquefasciatus with IC50 of 0.69â¯Î¼g/mL. The chemical structure of lawsone was identified based on spectroscopic analysis and literature spectral data. Insect-specific cysteine residues of AChE1 inhibitors will not interact with AChE1 of other organisms. Computational analysis showed that lawsone made strong binding interaction with cysteine residue in the AChE1 of Cx. quinquefasciatus. Hence, these results suggested that lawsone might act as a mosquito specific AChE1 inhibitor.
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Authors
Sylvester Darvin Sandhanam, Pathalam Ganesan, Antony Stalin, Gabriel Paulraj Michael, Kedike Balakrishna, Perumal Pandikumar, Savarimuthu Ignacimuthu, Abdullah Al-Dhabi Naif,