Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8405852 | Biocatalysis and Agricultural Biotechnology | 2018 | 44 Pages |
Abstract
Xylanases from bacterial sources have potential applications in various industrial processes. In the present study, the effect of enzymatic pre-treatment on pulp bleaching has been investigated using purified xylanase from alkaliphilic Bacillus licheniformis strain Alk-1. The xylanase produced by present isolate while growing on wheat bran was purified to homogeneity by 5.84-fold purification with ~15.97% recovery by anion exchange chromatography using DEAE-cellulose. The estimated molecular weight of the xylanase was ~46â¯kDa. The optimal pH and temperature for xylanase activity were pH 9.0 and 60â¯Â°C, respectively. The enzyme showed good activity retention (80%) after 1â¯h incubation at 60â¯Â°C and pH 9.0. Enzyme activity was stimulated by Ca2+, Fe2+ and Mg2+ and inhibited by Cd2+, Hg2+ and Cu2+ at 2â¯mM and 10â¯mM conc. For xylan, the enzyme gave a Km value of 5.26â¯mg/mL and Vmax value of 433â¯Î¼M/min/mg proteins when the reaction was carried out at 60â¯Â°Cand pH 9.0. Pre-treatment of kraft pulp with xylanase showed 19% reduction in kappa number, compared to control pulp, after 2â¯h treatment. The SEM and FTIR analysis of xylanase-treated pulp revealed significant morphological and structural changes on pulp fibres. GC-MS analysis of filtrates from control and xylanase-treated pulps showed variation in the presence of different plant-derived organic compounds. The present work finds potential application in paper production by making it cleaner and ecofriendly process.
Keywords
PAGEB. licheniformisPoly Acrylamide Gel ElectrophoresisDNSAg/LVmaxKBrRPMNaClCMCSDSGC-MSBSADEAE-celluloseIU/mlbovine serum albuminFourier transform infra redmaximum velocityHourWheat branSodium chloriderevolutions per minuteKappa numberFTIRGas chromatography mass spectroscopySEMmilliliterScanning electron microscopypotassium bromideoptical densityCarboxyl methyl celluloseGram
Related Topics
Life Sciences
Agricultural and Biological Sciences
Agricultural and Biological Sciences (General)
Authors
Abhay Raj, Sharad Kumar, Sudheer Kumar Singh, Jyoti Prakash,