Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8405993 | Biocatalysis and Agricultural Biotechnology | 2018 | 8 Pages |
Abstract
The phytopathogenic fungus Ceratocystis fimbriata produced an extracellular β-xylosidase when grown in mineral medium with wheat bran as carbon source. The enzyme was purified and characterized. The molecular mass was estimated by SDS-PAGE (161.6â¯kDa). The enzyme showed maximum activity at pH 3.9 and 65â¯Â°C, and it showed to be significantly thermostable at 60â¯Â°C, maintaining 70% of its maximum activity after 24â¯h of incubation at this temperature. The KM and VMAX using ÏNÏβXyl were 0.326â¯mM and 0.91 à 10â3 µmol/min, respectively. The β-xylosidase was partially inhibited by 1â¯mM of aluminum chloride, copper sulfate II, iron sulfate and SDS. The β-xylosidase combined with a xylanase had an additive effect on beechwood xylan hydrolysis, generating xylose as a final product. The supplementation of the enzymatic cocktail Multifect CL® with the β-xylosidase generated a more efficient hydrolysis of alkali-pretreated sugarcane bagasse when compared to the saccharification performed only by the cocktail Multifect CL®, showing an increase of 97.7% and 45.7% in glucose and xylose release, respectively.
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Authors
Marcele Pandeló Martins, Rafaela Zandonade Ventorim, Roberta Ribeiro Coura, Gabriela Piccolo Maitan-Alfenas, Rafael Ferreira Alfenas, Valéria Monteze Guimarães,