Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8406066 | Biocatalysis and Agricultural Biotechnology | 2018 | 33 Pages |
Abstract
Phytase is known to provide a solution for depletion of phosphorus (P). It helps it by hydrolyzing the insoluble P source in soil which is phytate. In this study, provides insight on yeast Candida tropicalis (NCIM 3321) which produces cell bound and extracellular thermostable phytase. The media components were optimized to enhance the enzyme production and checked for plant growth promoting activity. On optimization the isolate exhibited enhanced cell bound and extracellular phytase activity by four folds (from 236 to 1024 IU DCGâ1) and by five folds (from 0.46 to 1.95 IU mlâ1) respectively in 36Â h. The production time decreased to 24Â h compare to shake flask on Up-scaling the production process upto 10Â L scale, thus increasing the productivity of cell bound (1810 IU DCGâ1dayâ1) and extracellular phytase (6.08 IU mlâ1 dayâ1). The crude phytase (12 IU) from NCIM 3321 strain was studied for plant growth promotion activity in lab scale and field level experiments with maize crop. Findings of the study revealed that the extracellular phytase derived from non pathogenic C. tropicalis (NCIM 3321) was found to be plant growth stimulating by increasing the available P in soil. Our findings of phytase isolated from non-pathogenic yeast C. tropicalis NCIM 3321 exhibited dephytinization potential. Therefore, current study may have profound application in sustainable agriculture.
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Authors
Kumar Raja Puppala, Tejali Naik, Alfina Shaik, Syed Dastager, Ravi Kumar V, Jayant Khire, Mahesh Dharne,