Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8406197 | Biocatalysis and Agricultural Biotechnology | 2017 | 23 Pages |
Abstract
Pseudomonas sp. strain OCB-01 and Micrococcus sp. strain PCB-02 exhibiting dichlorobenzoic acid (diCBA) metabolic properties were isolated respectively from rotten onion bulb and polychlorinated biphenyl-contaminated soil by enrichment culture containing equivalent amounts of 2,5-diCBA and 3,4-diCBA. In addition to both diCBAs, organisms were subsequently found to also grow on 3-CBA, 4-CBA, and a wide range of aromatic and non-aromatic compounds. Growth on 2,5-diCBA and 3,4-diCBA resulted in two to four orders-of-magnitude cell increases with nearly 100% consumption of the substrate yielding an overall metabolic rate of 0.005Â mMÂ hâ1. Although strain OCB-01 appeared to utilize the congeners better, no statistical difference was observed in all incubations. The isolation of diCBA-degrading organism from rotten onion bulb is unprecedented, and this has again reinforced the fact that degraders of organopollutants may be widely distributed across diverse environmental systems than previously thought and may offer reasons for many failures in isolation of such organisms.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Agricultural and Biological Sciences (General)
Authors
Sunday A. Adebusoye, Olumuyiwa A. Adeosun, Bolanle B. Olofinlade,