Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8406218 | Biocatalysis and Agricultural Biotechnology | 2016 | 15 Pages |
Abstract
Oleaginous fungus Mortierella alpina 1S-4 accumulates arachidonic acid (20:4Ï6) as a major polyunsaturated fatty acid. However, 20:4Ï6 is not essential for the growth of M. alpina 1S-4, because various mutants that produce no 20:4Ï6 were isolated. M. alpina JT-180, a Î12 desaturation-defective mutant that was derived from M. alpina 1S-4 by treatment with a chemical mutagen, accumulates Mead acid (20:3Ï9) instead of 20:4Ï6. p-Anisidine was found to be a Î6 desaturation inhibitor in this study. The concentration of 0.1Â mg/ml of p-anisidine had an inhibitory effect on the growth of M. alpina JT-180. The addition of p-anisidine to the medium caused M. alpina JT-180 to produce only monoenoic acids such as oleic acid (18:1Ï9) and eicosenoic acid (20:1Ï9) as unsaturated fatty acids. The effects of exogenous fatty acids were investigated when M. alpina JT-180 was cultivated in medium containing p-anisidine. The addition of linoleic acid (18:2Ï6) and 6Z,9Z-octadecadienoic acid (18:2Ï9) restored the growth of M. alpina JT-180 cultivated on the medium containing p-anisidine, but palmitic acid (16:0), 18:1Ï9, and vaccenic acid (18:1Ï7) had no effect. For the growth of oleaginous fungus M. alpina, 18-carbon length fatty acids with more than two double bonds are considered to be essential.
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Agricultural and Biological Sciences (General)
Authors
Tomohiro Fujita, Hiroshi Kawashima, Eiji Sakuradani, Takaiku Sakamoto, Akinori Ando, Jun Ogawa, Sakayu Shimizu,