Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8408755 | The Crop Journal | 2018 | 10 Pages |
Abstract
Soybean is one of the world's most important oil and protein crops. Efficient transformation is a key factor for the improvement of soybean by genetic modification. We describe an optimized protocol for the Agrobacterium rhizogenes-mediated transformation of soybean and the induction of hairy root development in vitro. Cotyledons with 0.5-cm hypocotyls were cut from 5-day-old seedlings and used as explants. After infection and co-cultivation, hairy roots were produced in induction culture medium after 10-12Â days. Using this method, 90%-99% of the infected explants of five different cultivars produced hairy roots within one month. Observations using reporter constructs showed that 30%-60% of the hairy roots induced were transformed. Based on high transformation efficiency and short transformation period, this method represents an efficient and rapid platform for study of soybean gene function.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Agronomy and Crop Science
Authors
Li Chen, Yupeng Cai, Xiujie Liu, Chen Guo, Shi Sun, Cunxiang Wu, Bingjun Jiang, Tianfu Han, Wensheng Hou,