Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8424891 | Stem Cell Research | 2018 | 13 Pages |
Abstract
In summary, hPSC (hESC-HES3 and hiPSC-IMR90) can be efficiently differentiated to CMs in a scalable spinner process which integrates 7â¯days of expansion (3.01â¯Â±â¯0.51â¯Ãâ¯106 to 3.50â¯Â±â¯0.65â¯Ãâ¯106â¯cells/mL) followed by 10â¯days of WNT modulated CM differentiation and 5â¯days of lactate based purification. CM yield of 1.38â¯Â±â¯0.22â¯Ãâ¯106 to 1.29â¯Â±â¯0.42â¯Ãâ¯106â¯CM/mL with 72.5â¯Â±â¯8.35% to 83.12â¯Â±â¯8.73% cardiac troponin-T positive cells were obtained from these cultures.
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Authors
Sherwin Ting, Alan Lam, Gerine Tong, Allen Chen, Heming Wei, JianJun Wu, Yue Ning Lam, Shaul Reuveny, Steve Oh,