| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8424922 | Stem Cell Research | 2018 | 13 Pages |
Abstract
A skin biopsy was obtained from a 14-year-old female patient with a history of Myelomeningocele. Dermal fibroblasts were isolated and reprogrammed with Sendai virus (SeV) vectors encoding OCT3/4, SOX2, KLF4, and c-MYC. The generated induced Pluripotent Stem Cell (iPSC) clones NTDi4_09A were free of genomically integrated reprogramming genes, had a stable normal karyotype and expressed pluripotency markers. The iPSCs formed teratomas in mice, which were differentiated towards derivatives of the three germ layers in vivo. This iPSC line offers a useful resource to study a genetic profile of a patient with spina bifida.
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Authors
Hongran Wang, Shuying Zhao, Richard H. Finnell, Timothy George, Austin J. Cooney,