| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8424943 | Stem Cell Research | 2018 | 4 Pages |
Abstract
A 87-year old Alzheimer's Disease(AD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system was used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in AD.
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Authors
Ying Wang, Hongxiang Yu, Ying Chen, Gang Li, Ying Lei, Jian Zhao,