Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8425052 | Stem Cell Research | 2018 | 19 Pages |
Abstract
Primary arterial endothelial cell (AEC) is an attractive source of tissue-engineered blood vessels for therapeutic transplantation in vascular disease. However, scarcity of donor tissue, inability of proliferation and undergo de-differentiation in culture remain major obstacles. We derived a stable induced pluripotent stem cell (iPSC) line possessed all the characteristics of pluripotent state from human umbilical arterial endothelial cells by transduction of four human transcription factors (Oct4, Sox2, Klf4, and c-Myc) using sendai virus vectors. It will likely facilitate to lineage differentiate and generate sufficient AECs for clinical use in cardiovascular disease based on epigenetic memory of the tissue of origin.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Huilin Li, Haiyun Pei, Jiqin Nie, Mingyi Qu, Zeng Fan, Yali Jia, Lijuan He, Xue Nan, Wen Yue, Xuetao Pei,