Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8425389 | Stem Cell Research | 2018 | 5 Pages |
Abstract
NERCe002-A-3 cells were generated from the normal human embryonic stem cell line NERCe002-A. NERCe002-A-3 cells overexpressed 14-3-3ζ after exposure to doxycycline. 14-3-3ζ protein have the ability to bind a multitude of functionally diverse signalling proteins. The NERCe002-A-3 cell line is considered a model for functional studies of the 14-3-3ζ protein in hESC self-renewal and cell differentiation. Doxycycline-treated NERCe002-A-3 cells showed a > 27-fold increase in relative expression of 14-3-3ζ as compared with un-induced cells. Characterization assays proved that NERCe002-A-3 cells express typical markers of pluripotency and have the ability to form the three germ layers in vivo.
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Authors
Jingjing He, Xingxiang Duan, Wen Li, Yingying Peng, Juan Yu, Liang Hu, Sicong Zeng, Yang Wang, Guangxiu Lu, Ge Lin, Yi Sun,