Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8425429 | Stem Cell Research | 2018 | 5 Pages |
Abstract
Cystic Fibrosis (CF) is a monogenic, lethal disease caused by mutations in the cystic fibrosis transmembrane conductance (CFTR) gene. Here we report the production of CF-iPS cell lines from two different p.F508del homozygous female patients (Table 1). Two different primary cell types, skin fibroblasts and keratinocytes, were transfected with retroviral cocktails containing four: c-MYC, KLF4, OCT4 and SOX2 (MKOS) or three: KLF4, OCT4 and SOX2 (KOS) reprogramming factors. Two fibroblast-derived MKOS lines are described in the main text. The lines carry the p.F508del mutation, have a normal karyotype, express pluripotency markers and are able to differentiate into the three germ layers.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Aarne Fleischer, Iván M. Lorenzo, Esther Palomino, Trond Aasen, Fernando Gómez, Miguel Servera, VÃctor J. Asensio, VÃctor Gálvez, Juan Carlos Izpisúa-Belmonte, Daniel Bachiller,