Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8425565 | Stem Cell Research | 2018 | 5 Pages |
Abstract
The human embryonic stem cell line NERCe002-A-2 was generated by transduction of NERCe002-A cells with an expression vector carrying the luciferase gene. The stem cells labelled with luciferase can be transplanted into animals and detected by the bioluminescence imaging technology. This provides optimal prospects of application to in vivo stem cell tracing. Luciferin served as a substrate to detect the activity of luciferase, and luciferase expression was measured by quantitative PCR. Characterization assays suggested that the NERCe002-A-2 cell line expresses typical markers of pluripotency and can form the 3 germ layers in vivo.
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Authors
Yingying Peng, Menghan Xie, Xingxiang Duan, Liang Hu, Juan Yu, Sicong Zeng, Yang Wang, Qi Ouyang, Guangxiu Lu, Ge Lin, Yi Sun,