| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8425718 | Stem Cell Research | 2018 | 4 Pages |
Abstract
Pregnane X receptor (PXR) is a key nuclear receptor that mediates drug metabolism and stimulates hepatocyte proliferation. However, the lack of PXR expression in human pluripotent stem cell-derived hepatocytes limits their application for drug screening and toxicity testing. Here, we generated a PXR-mCherry reporter human induced pluripotent stem cell (hiPSC) line using the CRISPR/Cas9 system. PXR-mCherry hiPSCs were pluripotent and had differentiation potential and a normal karyotype. This cell line is an important tool for identifying factors that increase PXR-mediated drug metabolism and hepatocyte proliferation.
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Authors
Hyemin Kim, Ji-Woo Kim, So-Jin Kim, Young-Jun Choi, Dae-Sung Kim, Han-Jin Park,