| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8425742 | Stem Cell Research | 2018 | 4 Pages |
Abstract
Human immortalized Epstein-Barr virus (EBV) lymphoblastoids cells line (LCLs) from a 26-year- old male affected by an adult form of myotonic dystrophy type 1 (DM1) disease and carrying 200 CTG repeats mutation in the blood was used to generate induced pluripotent stem cells (iPSCs) using the Sendai virus expressing KLF4, OCT4, SOX2 and C-MYC. The resulting iPSCs were EBV free, expressed the pluripotency markers, could be differentiated into the three germ layers in vitro, had a normal karyotype, and retained the genetic DM1 mutation. This iPSC line could be useful for the investigation of DM1 mechanisms.
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Authors
Laurie Martineau, Véronique Racine, Siham Ait Benichou, Jack Puymirat,