Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8425744 | Stem Cell Research | 2018 | 4 Pages |
Abstract
We discuss the reprogramming of CD34+ cells isolated from UCB of a healthy female child of Indian ethnicity. The CD34+Â cells were nucleofected using episomal vectors expressing Oct4, Sox2, L-Myc, Klf4, Lin28 and p53DD (negative mutation in p53). The colonies were stained for alkaline phosphatase and evaluated for pluripotency marker expression by PCR, immunofluorescence and flow-cytometry. The safety of cells was confirmed by absence of plasmid in subsequent passages by PCR. G-banded karyotype demonstrated a stable genome. The ability of tri-lineage differentiation was confirmed by specific marker expression by immunofluorescence invitro and teratoma formation invivo.
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Authors
Sophia Fernandes, Prajakta Shinde, Nikhat Khan, Sanjay Singh, Shakti Vardhan, Velu Nair, Vaijayanti Kale, Lalita Limaye,