Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8425777 | Stem Cell Research | 2017 | 5 Pages |
Abstract
A human iPSC line was generated from exfoliated renal epithelial (ERE) cells of a patient affected with Congenital Heart Disease (CHD) and Laterality Defects carrying tshe variant p.R152H in the DAND5 gene. The transgene-free iPSCs were generated with the human OSKM transcription factor using the Sendai-virus reprogramming system. The established iPSC line had the specific heterozygous alteration, a stable karyotype, expressed pluripotency markers and generated embryoid bodies that can differentiate towards the three germ layers in vitro. This iPSC line offers a useful resource to study the molecular mechanisms of cardiomyocyte proliferation, as well as for drug testing.
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Authors
Fernando Cristo, José M. Inácio, Graça Rosas, Isabel Marques Carreira, Joana Barbosa Melo, LuÃs Pereira de Almeida, PatrÃcia Mendes, Duarte Saraiva Martins, José Maio, Rui Anjos, José A. Belo,