Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8427433 | Theriogenology | 2018 | 25 Pages |
Abstract
The best result was obtained by dropping directly into liquid nitrogen 20â¯Î¼l of spermatozoa suspension (drop-wise method) diluted with Mounib buffer containing 10% Me2SO + 10% glycerol. The addition of a mixture of anti-freezing proteins, AFPI and AFPIII, to Mounib buffer significantly increases the spermatozoa quality following vitrification so confirming the usefulness of AFPs in improving the quality of gametes subjected to the vitrification process. The present study proves that vitrification offers an alternative to conventional sperm cryopreservation also in this species.
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Authors
Loredana Zilli, Annalisa Bianchi, Maroua Sabbagh, Laura Pecoraro, Roberta Schiavone, Sebastiano Vilella,