Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8464112 | Cryobiology | 2018 | 4 Pages |
Abstract
Isochoric (constant volume) freezing has been recently suggested as a new method for cell and organ preservation. As a first step in studying the effect of isochoric freezing on mammalian cells, Madin-Darby canine kidney epithelial cells (MDCK), were frozen in an isochoric system, in a simple extracellular phosphate buffered solution to â10 °C (96.5â¯MPa), - 15 °C (162â¯MPa) and â20 °C (205â¯MPa) for 60 and 120â¯min. Cell membrane integrity and cell metabolism were studied with a Live/Dead cell vitality assay and flow cytometry. We found that cell survival decreases with an increase in pressure (lower temperatures) and time of exposure. For example, 60% of cells survived 60â¯min at - 10 °C and only 18% survived 120â¯min at this temperature. Negligible survival was measured at - 20 °C. This study may serve as the baseline towards further research on techniques to optimize the effects of isochoric freezing on living biological matter.
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Agricultural and Biological Sciences (General)
Authors
J. Preciado, B. Rubinsky,