Article ID Journal Published Year Pages File Type
8483576 Journal of Equine Veterinary Science 2016 23 Pages PDF
Abstract
The objective of this study was to determine the effectiveness of oxygen removal in minimizing oxidative stress to improve fertile longevity of equine spermatozoa. Stallion ejaculates (n = 6) were treated with increasing doses of the oxygen scavenger Oxyrase (0.6 U/mL, 1.2 U/mL, 2.4 U/mL, and 5.0 U/mL). Samples were cryopreserved, thawed, and analyzed for motility over 24 hours at ambient temperature. These data suggest a role of excessive oxygen in the reduced motility of cryopreserved samples as 2.4 U/mL Oxyrase minimized the loss of post-thaw motility over the other doses and control treatments. Oxygen, a key component of reactive oxygen species generation, is directly involved in oxidative damage, and its removal may trigger metabolic changes that are more suited for post-thaw motility maintenance. Removal of oxygen from semen before cryopreservation may promote increased post-thaw fertility and may have the potential to overcome stallion variability in cryosurvival.
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Life Sciences Agricultural and Biological Sciences Animal Science and Zoology
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