Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8493015 | Aquaculture | 2018 | 6 Pages |
Abstract
This study evaluated the use in vitro and in vivo of Bixa orellana seed extracts on the hematological and biochemical parameters of Colossoma macropomum co-infected with bacterium and monogenean, Anacanthorus spathulatus. The extract presented antimicrobial and antiparasitic activity in vitro against the pathogens. Fish supported the toxicity test and in vivo assay used 180 fish distributed in six treatments in triplicate: non-parasitized fish non-injected with A. hydrophila; non-parasitized fish non-injected exposed to acetone 0.2%; parasitized fish injected non-treated with the extract; parasitized fish injected treated with 125â¯Î¼gâ¯mLâ1 of extract in 2â¯h bath for two consecutive days; parasitized fish injected treated with 250â¯Î¼gâ¯mLâ1 of extract in 2â¯h bath for two consecutive days; parasitized fish injected treated with 125â¯Î¼gâ¯mLâ1 of extract for 12â¯h. After last bath, the fish were examined. Acetonic extract showed minimal inhibitory concentration of 125â¯Î¼gâ¯mLâ1 against A. hydrophila in the in vitro test and 100% efficacy against monogenean with therapeutic baths in relation to control non-treated in the in vivo test. Aeromonas hydrophila infection did not cause mortality. The gross pathology analysis showed ascites and hemorrhagic liver, kidney and spleen. Increased hemoglobin concentration, mean corpuscular hemoglobin concentration and total number of erythrocytes was followed by a decrease in hematocrit percentage in non-treated fish compared to basal group. Lymphocytes from fish in the control group were higher than in the other groups. Glucose was higher in treated fish than that found in those of basal and control. The results demonstrated that B. orellana bath was an effective alternative to treat fish diseases.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Aquatic Science
Authors
Jaqueline Inês Alves de Andrade, Gabriela Tomas Jerônimo, Cecilia Verônica Nunez, Raphael Brito dos Santos, Jandiara Kelly Oliveira Araújo, Maria Luiza Ruiz, José Luiz Pedreira Mouriño, Vanessa Neves Carvalho Santos, MaurÃcio Laterça Martins,