Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8493107 | Aquaculture | 2018 | 12 Pages |
Abstract
In intraperitoneal germ-cell transplantation techniques in salmonids, donor-derived diploid germ cells proliferated and differentiated within triploid recipient gonads, and the surrogate recipients produced only donor-derived gametes, which were capable of generating viable offspring when used in fertilization. To date, germ-cell transplantation techniques have been developed for use in perciforms, including sciaenids, however, triploid recipients have not yet been utilized in marine fishes. In this study, triploid Nibe croaker was produced at high triploidization rates (~Â 100%) using cold-shock treatments. Analysis of the gonadal development and gametogenesis of triploid Nibe croaker in two consecutive spawning seasons revealed that the triploid Nibe croaker was functionally sterile in both sexes and that the triploids would be suitable for use as surrogate recipients.
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Authors
Yutaka Takeuchi, Takashi Yatabe, Hiroyuki Yoshikawa, Yasuko Ino, Naoki Kabeya, Ryosuke Yazawa, Goro Yoshizaki,