Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8493175 | Aquaculture | 2018 | 5 Pages |
Abstract
The red seabream (Pagrus major) is an economically important marine fish species that is cultured in Japan. Natural outbreaks of red seabream iridovirus (RSIV) and/or Streptococcus iniae can cause massive mortalities and severe economic losses in red seabream production. No effective single-injection vaccine for protecting red seabream against both diseases has yet become commercially available. Here, we evaluated the efficacy of a bivalent formalin-killed vaccine (PISCIVAC⢠Irido Si) against RSIV and S. iniae infections in red seabream. In laboratory trials, intraperitoneally vaccinated fish reared at 25â¯Â°C were protected against challenges with RSIV and S. iniae as early as 3â¯days post vaccination (dpv), with a relative percent survival (RPS) of 69% and 90%, respectively. The protection was also observed in fish reared at 15â¯Â°C, with a RPS of 88% and 89% after challenging the fish with RSIV and S. iniae at 10â¯dpv and 14â¯dpv, respectively. Furthermore, intramuscular vaccination effectively protected fish against RSIV (RPS of 100% and 65-96% at 10 and 20â¯dpv, respectively) and S. iniae (RPS of 92-100% at 14â¯dpv). The vaccine conferred protection against RSIV infection for at least 2â¯months and against S. iniae infection for at least 6â¯months in the field trial. These results indicate that PISCIVAC⢠Irido Si is effective for protecting red seabream against RSIV and S. iniae infections.
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Authors
Kittipong Thanasaksiri, Kohei Fukuda, Shiori Tsubone, Hidenori Miyadai, Taiga Murakami, Ayana Murakami, Ryoko Takano,