Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8493300 | Aquaculture | 2018 | 26 Pages |
Abstract
Standing duration (from fertilization to the beginning of cold shock), shocking temperature, and cold shock duration are critical factors for improving the fertility of the female parent and survivability of offspring in the gynogenesis of topmouth culter (Culter alburnus). A 3-factor quadratic-orthogonal-rotation combination design experiment was conducted to evaluate the main and interacting effects of these three factors on gynogenesis efficiency with the fewest experimental combinations. Two indices including gynogenesis and hatching rates were measured repeatedly for each experimental combination. Using quadratic regression equations of the two experimental indices for these factors, optimal factor combinations were screened within factorial-level intervals, such as a standing duration of 6â¯min, shock duration of 18â¯min, and shock temperature of 7â¯Â°C for the gynogenesis rate, while 6â¯min, 23â¯min, and 5â¯Â°C, respectively, were identified for the hatching rate. Response surface analysis showed that within factorial intervals, the single-factor curve and double-factor surface exhibited a convex shape. Among the three factors, standing duration showed the greatest contribution to both gynogenesis and hatching rates, while shocking duration showed the smallest contribution.
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Authors
Ting Li, Yongyi Jia, Jin Gao, Jingli Zhao, Wenping Jiang, Zhimin Gu, Runqing Yang,