Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8493358 | Aquaculture | 2018 | 39 Pages |
Abstract
Mono-sex culture of yellow drum (Nibea albiflora) provides economic advantages in industry because of the superior growth of females relative to males. Sex-reversal of genetic females (XX) to produce neo-males is an indispensable step toward establishing all-female populations. To achieve this goal, gynogenetic yellow drum at an undifferentiated sex stage (total length, 1.63â¯Â±â¯0.16â¯cm) were immersed in 17α-methyltestosterone (17α-MT) for 2â¯h/day from 30 to 90â¯dph (60â¯days), producing sex-reversed fish (neo-males). All the four 17α-MT concentrations tested (0.2, 2, 10, 50â¯Î¼g/L) drove the development of testes, evidenced by histological observations of the gonads. The masculinization of gynogenetic yellow drum was also demonstrated by the expression patterns of two sex-specific genes, dmrt1 and cyp19a1a: the gonads of 17α-MT-treated fish exclusively expressed male-specific dmrt1 with no expression of female-specific cyp19a1a. Specifically, treatment with 0.2â¯Î¼g/L 17α-MT yielded complete sex-reversed fish without any abnormalities in gonadal development, and the growth of neo-males was not significantly different to that of controls during the treatment period. However, testicular abnormalities were observed in groups treated with higher 17α-MT concentrations, with a trend for more abnormalities with increasing 17α-MT concentrations, and the growth of 17α-MT-treated fish was retarded at these higher 17α-MT concentrations. These results indicated that high concentrations of 17α-MT had adverse effects on growth and gonadal development, and the most efficient concentration tested for producing neo-males was 0.2â¯Î¼g/L. Furthermore, crossing of gynogenetic sex-reversed males with normal females produced 100% females, demonstrating the potential efficiency of this protocol for reversing the sex of yellow drum and establishing large-scale production of all-female populations.
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Authors
Dongdong Xu, Fang Yang, Ruiyi Chen, Bao Lou, Wei Zhan, Takao Hayashida, Yutaka Takeuchi,