Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8493541 | Aquaculture | 2018 | 36 Pages |
Abstract
In a 16-week feeding trial, the effects of dietary vitamin E (α-tocopherol, α-Toc) levels on tissue α-Toc concentration, hematology and non-specific immune responses of Japanese eel were examined. Totally five diets were used in this experiment, consisting a basal commercial diet (control) and supplementing 100, 150, 200 or 400 mg α-Toc kgâ 1 diet in the form of dl-α-tocopherol acetate. The analyzed α-Toc concentrations of the diets were 32, 112, 158, 212 and 428 mg kgâ 1 diet, respectively. For each experimental diet, triplicate groups of fish (initial body weight, 360 g) were fed at a ratio of 2% body weight. At the end of the feeding trial, α-Toc concentration in liver, kidney, muscle and gonad were increased significantly in a dose dependent manner (P < 0.05). Significantly higher hemoglobin and white blood cells count were found in fish fed 212 mg α-Toc kgâ 1 diet compared to control. Plasma glucose concentration was decreased significantly in all α-Toc supplemented groups, also significantly higher aspartate aminotransferase and alanine aminotransferase values were obtained at 32 and â¤Â 158 mg α-Toc kgâ 1 diet compared to the other groups. Significantly higher lysozyme and SOD activities were recorded in fish fed 428 mg α-Toc kgâ 1 diet compared to the other groups. The optimal dietary α-Toc requirement was estimated at 212.9 mg α-Toc kgâ 1 diet by broken-line regression model based on liver α-Toc concentration.
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Agricultural and Biological Sciences
Aquatic Science
Authors
Erfan Shahkar, Ali Hamidoghli, Hyeonho Yun, Dae-Jung Kim, Sungchul C. Bai,