Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8493550 | Aquaculture | 2018 | 12 Pages |
Abstract
The pesticide chlorpyrifos-methyl (CLP-m) has been identified in plant ingredients intended for aquaculture feed production. To investigate systemic effects of CLP-m with emphasis on lipid metabolism, post-smolt Atlantic salmon were fed in duplicate (n = 2) either diets with no CLP-m (Control) or CLP-m at different concentrations (0.1, 1.0 or 8.0 mg kgâ 1) for a total of 67 days (Low, Medium, High). Fish in all groups almost doubled their weight during the feeding trial from 262 ± 26 g (mean ± SD) to 465 ± 64 g (overall mean), with no significant effects on any growth parameters. There was a significant dose-dependent inhibition of plasma cholinesterase activity (BuChE) after 67 days. The CLP-m biotransformation metabolite, TCP was detected in liver and bile, with low levels of the parent compound in the organs. Spleen somatic index decreased significantly with increasing dietary CLP-m intake. Hematocrit (%) decreased linearly with increasing dietary exposure to CLP-m after 30 days of exposure, but this decrease was less at 67 days of exposure. A significantly reduced content of arachidonic acid (ARA 20:4n â 6), accompanied by a significantly increased content of the saturated fatty acid, palmitic acid (PA 16:0), was observed in liver phospholipids (PLs) with increasing dietary content of CLP-m. Major effects were seen on the PL classes in liver which showed a significantly decreased absolute content, possibly indicating inhibition of PL remodeling pathways or other membrane perturbation effects from CLP-m exposure. In conclusion, this study shows that the pesticide CLP-m is a relatively potent toxicant in Atlantic salmon, especially affecting liver PLs and ARA metabolism.
Keywords
MRLethoxyresorufin-O-deethylasePLSbutyrylcholinesteraseBuChEERODSSIRBCOCPsPesticideOrganochlorine pesticidesAChEOrganophosphateAcetylcholinesteraseArachidonic acidMRIMagnetic resonance imagingFeedingMaximum residue limitARACondition factorphosphatidylcholinePhospholipidsAtlantic salmonLipid metabolismChlorpyrifos-methylChlorpyrifosred blood cell
Related Topics
Life Sciences
Agricultural and Biological Sciences
Aquatic Science
Authors
M. Sanden, P.A. Olsvik, L. Søfteland, J.D. Rasinger, G. Rosenlund, B. Garlito, M. Ibáñez, M.H.G. Berntssen,