In vivo metabolism of unsaturated fatty acids in Sepia officinalis hatchlings

While designing an inert diet, C18 FA and LC-PUFA levels and ratios should also be considered, since the esterification pattern detected in the present study suggested a competition between these FA for esterification into specific lipid classes. Moreover, considering the observed esterification pattern of LC-PUFA into different lipid classes, it is likely that the DHA/EPA/ARA ratio, rather than DHA/EPA or EPA/ARA ratios, would be of great importance for S. officinalis hatchling development.