Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8496246 | Aquaculture | 2008 | 8 Pages |
Abstract
Seventy nine microsatellite markers selected across all linkage groups (LG) from a previous turbot genetic map were studied in a diploid meiogynogenetic family for centromere mapping using half-tetrad analysis. Significant deviations from Mendelian segregation were observed at 25% loci analyzed. The clustering of distorted loci at specific LGs, suggested the existence of genes of different deleterious effects. The lack of Mendelian segregation distortion at 1Â day and 10Â days post-hatching larvae at these loci precluded an explanation based on aberrant meiotic segregation. Heterozygote frequency distribution in gynogenetic offspring showed close to 50% values above 0.667, which suggested high chiasma interference in turbot. Complete interference appeared as the best fitting function when estimating centromere position. However, Kosambi and Haldane functions performed better at specific LGs as a consequence of the variable crossover pattern of centromere-distant markers among LG. Great concordance between half-tetrad data and the positions previously reported in the turbot map was observed. Most centromeres were localized with an error around or below 5Â cM and closely linked markers exist now in 8 LGs. Centromere location was mostly in accordance with previous karyotypic information.
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Authors
Paulino MartÃnez, Miguel Hermida, Belén G. Pardo, Carlos Fernández, Jaime Castro, Rosa M. Cal, José A. Álvarez-Dios, Antonio Gómez-Tato, Carmen Bouza,