Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8506108 | Veterinary Parasitology | 2018 | 5 Pages |
Abstract
The aims of this study were: 1) to assess the anthelmintic effect of Gliricidia sepium on the establishment of C. punctata third-stage larvae (L3) in calves, and 2) to isolate and to elucidate an anti-exsheathment phytochemical from the plant offered during the trial. Twelve ¾ Holsteinâ¯Ãâ¯Zebu calves were divided in two experimental groups: control (T1) and treatment (T2) (nâ¯=â¯6). After adaptation, each calf was infected with an oral dose of 400 C. punctata L3/Kg LW. Basal diet consisted of Digitaria decumbens hay (6.27% CP) and commercial concentrate (12% CP). In addition, during the experimental period T2 received fresh G. sepium leaves (26.88% CP) ad libitum. On day 9 post-infection, three calves per treatment were randomly selected for slaughter, and worm counts were performed. Larval establishment rates obtained were 13.44â¯Â±â¯0.13% and 3.1â¯Â±â¯1.42% for T1 and T2, respectively (Pâ¯<â¯.05). The reduction of larval establishment was 76.9%. The total length of worms recovered from the animals was also affected by the intake of G. sepium (Pâ¯<â¯.05). Phytochemicals present in G. sepium leaves offered to calves were isolated through silica gel columns and elucidated through Magnetic Nuclear Resonance (1H and 13C). Bio-guided isolation procedures lead to the elucidation of Oxytroside (Kaempferol 3-O-rhamnopyranosyl-(1â¯ââ¯6)-β-D-glucopyranoside-7-O-rhamnopyranoside), which fully inhibited the C. punctata exsheathment process (2400â¯Î¼gâ¯mLâ1). Gliricidia sepium represents an alternative to prevent severe C. punctata infections by reducing larval establishment in cattle.
Keywords
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Animal Science and Zoology
Authors
von Son-de Fernex Elke, Alonso-DÃaz Miguel Ángel, Valles-de la Mora Braulio, Mendoza-de Gives Pedro, Castillo-Gallegos Epigmenio, Zamilpa Alejandro, González-Cortazar Manases,