Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8506171 | Veterinary Parasitology | 2018 | 6 Pages |
Abstract
Neospora caninum is a member of Apicomplexa phylum, the causative agent of neosporosis. The neosporosis combat is not well established and several strategies related to vaccine, chemotherapy and immune modulation are under development. In this work, we evaluated the effects of artemisinin (Art), methylene blue (MB) and pyrimethamine (Pyr) alone or associated, on N. caninum proliferation and elimination using LacZ tagged tachyzoites. The reactive oxygen species (ROS) production after incubation with Art were also performed. Our results indicate that combinations of classical antimalarial drugs improve the parasite control, allowing the use of three drugs in a single dose. Additionally, artemisinin demonstrated distinct ROS production patterns in intra and extracellular N. caninum forms. The drug repurposing appears as a suitable approach, allowing a fast and safe method to evaluate old drugs but novel candidates against neosporosis.
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Authors
Luiz Miguel Pereira, Gabriela de Luca, Nathália de Lima Martins Abichabki, Cássia Mariana Bronzon da Costa, Ana PatrÃcia Yatsuda,