Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8507507 | Ticks and Tick-borne Diseases | 2018 | 4 Pages |
Abstract
The development of transgenic techniques has been reported in many protozoan parasites over the past few years. We recently established a successful transient transfection system for Babesia gibsoni based on Bg 5â²-ef-1α promoter. This study investigated 6 homologous and 6 heterologous promoters for B. gibsoni and B. bovis and identified novel interchangeable cross-species functional promoters between B. gibsoni and B. bovis. Ten out of twelve promoters had heterologous promoter function. In particular, Bg 5â²-ef-1α and Bg 5â²-actin heterologous promoters resulted in a significantly higher luciferase activity than Bb 5â²-ef-1α homologous promoter in B. bovis. The present study showed that Bg 5â²-actin promoted the highest luciferase activity in both B. gibsoni and B. bovis. The study further indicates that heterologous promoter function widely exists between B. gibsoni and B. bovis. This finding is an important step for future stable transfection construct design and for the production of vaccines based on transfected B. gibsoni and B. bovis parasites.
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Authors
Mingming Liu, Paul Franck Adjou Moumouni, Shinuo Cao, Masahito Asada, Guanbo Wang, Yang Gao, Huanping Guo, Jixu Li, Patrick Vudriko, Artemis Efstratiou, Aaron Edmond Ringo, Seung-Hun Lee, Hassan Hakimi, Tatsunori Masatani, Fujiko Sunaga,