Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8634871 | Agri Gene | 2018 | 5 Pages |
Abstract
At altitudes >1500â¯m, measurements of mean pulmonary arterial pressures (mPAP; mmHg) are a measure of pulmonary hypertension (PH) in cattle. Genotypes of a G/A single nucleotide polymorphism (SNP; rs208684340) in the endothelial PAS domain protein 1 (EPAS1) gene were determined for mPAP categories (low, moderate, high) on Angus cattle. The A allele of this SNP was postulated to be associated with altitude-induced PH. The first objective was to estimate allele and genotypic frequencies in Angus cattle at high- (elevation 1850 to 2800â¯m) and low-altitude (elevation 91 to 1192â¯m) ranches (nâ¯=â¯118; random sample of 1275). Percent of cattle at these low elevation ranches with genotypes G/G, G/A, and A/A were 64.4, 33.9, and 1.7%, respectively, and a minor allele frequency (MAF; A allele) of 18.6%. Bulls, steers, and heifers (nâ¯=â¯691) from 4 high-altitude Angus herds in Colorado and Wyoming were genotyped. Percent of cattle with genotypes G/G, G/A, and A/A were 48.6, 34.3, and 17.1%, respectively, with a MAF of 34.2%. Average mPAP was 40.5â¯Â±â¯8.8â¯mmHg. Low, moderate, and high-mPAP category MAF were similar (Ï2â¯=â¯3.03; Pâ¯=â¯0.22), with values of 35.4, 31.0, and 37.8%, respectively. Additionally we sought to evaluate the genotype-to-phenotype association of this SNP with mPAP phenotypes (nâ¯=â¯532) from the Colorado State University Beef Improvement Center (elevation 2150â¯m) Angus herd. Linear mixed model analysis suggested genotype was not a predictor (Pâ¯=â¯0.61) of mPAP. Results do not suggest the A allele of SNP in EPAS1 is associated with high altitude-induced PH in yearling Angus cattle.
Keywords
mPAPN-terminal transactivation domainODDDN-TADRHFEPAS1HIFHWENTCAngusanalysis of varianceANOVATris-EDTAHardy-Weinberg equilibriumoxygen-dependent degradation domainHypoxia-induced factorPulmonary arterial pressuremean pulmonary arterial pressureRight heart failurepolymerase chain reactionPCRPulmonary hypertensionSingle nucleotide polymorphismSNPquality controlCattle
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Authors
N.F. Crawford, S.J. Coleman, T.N. Holt, S.E. Speidel, R.M. Enns, R. Hamid, M.G. Thomas,