Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8634893 | Agri Gene | 2018 | 35 Pages |
Abstract
Late blight (LB), Phytophthora infestans (PI) as pathogen, is the most devastating disease limiting potato production globally. In the Tropic highlands it can reduce yields by >50%. Cooperation-88 (C88) is a potato variety with high levels of durable resistance to PI, even after being widely grown for over 20â¯years. To more fully understand the mechanism of the resistance to PI of this variety, we inoculated detached leaves of young C88 plants with PI isolate XA-4 (super race with vir 1-11). Samples of one day post pathogen inoculation (1 dpi), 3 dpi, 5 dpi and control treated with sterile water were collected and frozen with liquid nitrogen. Transcriptomes of these 4 samples were sequenced by the next-generation sequencing platform Illumina Hiseq 2000. Totally ~68â¯M clean reads were obtained. With the genome of S. phureja clone DM1-3 516R44 as reference, these clean reads were processed by GO analysis, KEGG pathway enrichment analysis and differential expression analysis. PI inoculation of C88 leaves resulted in the following: a) in the 3 pathogen inoculated samples, 3950 were differentially regulated compared to those in the control; c) at 1 dpi increased expression genes were nearly double the number of decreased expression genes; d) 665 genes were differentially expressed in every treatments; e) gene expression levels in photosynthesis and the chloroplast were mostly diminished. The high durable late blight resistance C88 is composed of: a) 344 expressed R genes; b) 9 pathogenesis-related genes, particularly osmotin gene and endochitinase gene expressions increased dramatically with PI inoculation; c) thirty genes for phenolic compounds synthesis expressions increased with PI inoculation from small to large multiples compared to the control; and d) R genes need pathogenesis proteins and phenolic compounds to have superior durable resistance to PI.
Keywords
DAHPPhytophthora infestansDPIAATEPSPsAOCRPKMPLA1R genesPEPC88Anthocyanin acyltransferaseR geneE4Popr33GT3-deoxy-d-arabino-heptulosonate-7-phosphateANPCHSF3Hflavonoid 3′-hydroxylaseallene oxide cyclaseErythrose 4-phosphatejasmonic acidLOXAOSPhenolic compoundsKEGG یا Kyoto Encyclopedia of Genes and Genomes Kyoto Encyclopedia of Genes and GenomesLate blightANSanthocyanidin synthaseAllene oxide synthasechalcone synthasePotatoSAPJARphosphoenolpyruvatePhospholipase A1lipoxygenaseAGTPathogenesis-related proteinPathogenesis-related proteinsResistance gene
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Authors
Dahai Hao, Jie Yang, Weibiao Long, Jing Yi, Peter VanderZaag, Canhui Li,