Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8647744 | Plant Gene | 2018 | 31 Pages |
Abstract
Dehydrins (DHNs) are highly hydrophilic, thermo stable, calcium dependent chaperons involved in plant developmental processes as well as in diverse abiotic stresses. A systematic survey resulted in the identification of 7 dehydrins (DHNs) in Setaria italica and Zea mays, but 6 in Sorghum bicolor. They are classified into 5 sub-groups, namely YnSKn, SKn, KnS, S, and YnS. DHNs of Sorghum exhibit 1 ortholog with Oryza sativa and Z. mays and 3 with S. italica. Unlike other DHNs, SbDHN5 has been found as an ordered protein with many phosphorylation sites. Network analyses of novel YnS subgroup showed interaction with HSP70 and FKBP genes. In silico promoter analysis revealed the presence of abscisic acid (ABA), drought, salt, low temperature stress-responsive elements. The miRNA target analysis revealed DHNs are targeted by 51 miRNAs responsive to abiotic stresses. High transcript expressions of DHNs were observed in root, stem and leaf compared to inflorescence in S. bicolor. All DHN genes exhibited high levels of expression in stem under cold, heat, salt, and drought stresses. In contrast to other DHNs, the SbDHN2 of YnS subgroup, exhibited the highest expression, under multiple stresses in all the tissues indicating its involvement against a wide array of abiotic stresses.
Keywords
ILPUTRsLTRDREIDPPKCDehydrinspKaqRT-PCRLEAHSESSRmicro RNAAbiotic stressABRESimple sequence repeatsLate embryogenesis abundant proteinsPhosphorylation sitesSorghumMiRNAneighbour joiningquantitative real-time PCRMolecular weightsIntrinsically disordered proteinsprotein kinase AProtein kinase Ccasein kinase
Related Topics
Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
M. Nagaraju, Palakolanu Sudhakar Reddy, S. Anil Kumar, Anuj Kumar, Prashanth Suravajhala, Altaf Ali, Rakesh K. Srivastava, P.B. Kavi Kishor, D. Manohar Rao,